Technical and Clinical Characterization of the Bio-PTH (1–84) Immunochemiluminometric Assay and Comparison with a Second-Generation Assay for Parathyroid Hormone
نویسندگان
چکیده
منابع مشابه
Experience with a third-generation parathyroid hormone assay (BIO-PTH) in the diagnosis of primary hyperparathyroidism in a Brazilian population.
Objective To evaluate the usefulness of a third-generation PTH assay in the diagnosis of primary hyperparathyroidism (PHPT). Subjects and methods Forty-one PHPT patients (4 men and 37 women) with 61.2 ± 10.9 (mean ± SD) years, were studied and had PTH levels measured with two different methods using the same immunochemiluminescent assay plataform (Elecsys 2010 System, Roche). We compared a se...
متن کاملRapid PTH assay by simple modification of Nichols intact PTH-Parathyroid Hormone Assay Kit.
In recent years, there has been an interest in producing a rapid, intraoperative assessment of parathyroid hormone (PTH) in serum to evaluate the efficacy of para-thyroid surgery [1-3]. Here, we report a simple modification of the intact PTH-parathyroid hormone overnight assay from Nichols Insutute Diagnostics (San Juan Capistrano, CA) that is usable by laboratories having Abbott (Abbott Park, ...
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15 صفحه اولMeasuring Parathyroid Hormone (PTH) in Patients with Oxidative Stress – Do We Need a Fourth Generation Parathyroid Hormone Assay?
Oxidation of PTH at methionine residues results in loss of biological activity. PTH may be oxidized in patients with renal disease. The aim of this study was to develop an assay considering oxidation of PTH. Oxidized hPTH was analyzed by high resolution nano-liquid chromatography coupled to ESI-FTT tandem mass spectrometry (nanoLC-ESI-FT-MS/MS) directly and after proteolytic cleavage. The oxidi...
متن کاملParathyroid-hormone-related peptide immunochemiluminometric assay. Developed with polyclonal antisera produced from a single animal.
An immunochemiluminometric assay of parathyroid-hormone-related peptide (PTHrP) was developed with purified antisera produced from a single goat immunized with (1-86) PTHrP. One batch of purified antibodies was labeled with acridinium ester used as tracer antibodies; a second batch of the purified antibodies was immobilized onto plastic bead. Sensitivity of the assay was 0.1 pmol/L. The assay h...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2004
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2003.026831